Pharmaceutical Microbiology MCQ: 30 Best Free Questions with Answers

Pharmaceutical microbiology MCQ questions are a mix of history, staining, sterilisation and disease-causing organisms. This quiz has 30 questions that cover all four areas, so you can find out quickly which one needs more revision.

Microbiology also helps in sterile product manufacturing and quality control, so it is worth learning well.

Try the Pharmaceutical Microbiology MCQ quiz first

Answer the 30 questions below one by one. You will see whether each answer is right as you go, so keep a pen and paper ready to note the ones you miss.

Pharmacy Quiz 9 - Pharmaceutical Microbiology

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The first person to observe bacteria under a microscope was

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Penicillin was discovered by

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The smallpox vaccine was developed by

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The postulates for linking a microbe with a disease were given by

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Gram staining was developed by

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Gram-positive bacteria appear after Gram staining as

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The cell wall of Gram-positive bacteria is rich in

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The outer membrane of Gram-negative bacteria contains

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Which of these is a Gram-positive coccus occurring in clusters?

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The causative organism of tuberculosis is

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The stain used to identify Mycobacterium tuberculosis is

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The causative organism of cholera is

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The causative organism of typhoid fever is

14 / 30

The malaria parasite belongs to the genus

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The biological indicator used to test autoclave sterilization is

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The biological indicator used for dry heat sterilization is

17 / 30

The phenol coefficient test is used to evaluate the

18 / 30

The most commonly used solidifying agent in culture media is

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Nutrient agar is a

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Bacteria reproduce mainly by

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The bacterial capsule mainly protects the cell from

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Bacterial flagella are mainly responsible for

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A plasmid is an

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The genetic material of a virus is

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HIV infection leads to

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The oral polio vaccine was developed by

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The BCG vaccine gives protection against

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Penicillin is produced by the fungus

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Streptomycin is produced by

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The Minimum Inhibitory Concentration (MIC) is the

Your score is

The average score is 0%

Why Pharmaceutical Microbiology deserves your time

Most students find microbiology easy to understand but hard to remember. There are many names of organisms, scientists and diseases. The trick is to group them: one list for scientists and their discoveries, one list for organisms and their diseases, and one list for sterilisation and indicators. When you revise group by group, the facts stop mixing.

Tricky Pharmaceutical Microbiology MCQ questions explained

These are the questions from the set above that students most often get wrong, with the reason behind each answer.

  1. Who first saw bacteria under a microscope?
    Answer: Anton van Leeuwenhoek. Pasteur, Koch and Fleming made their discoveries later.
  2. What colour do Gram-positive bacteria show after Gram staining?
    Answer: Purple or violet. Their thick peptidoglycan wall retains the crystal violet. Gram-negative bacteria appear pink.
  3. Which stain is used to identify tuberculosis bacilli?
    Answer: Ziehl-Neelsen (acid-fast) stain. Gram staining does not work well on mycobacteria because of their waxy wall.
  4. Which organism is the biological indicator for autoclave sterilisation?
    Answer: Geobacillus stearothermophilus. For dry heat sterilisation, Bacillus subtilis var. niger is used.
  5. What does MIC stand for and mean?
    Answer: Minimum Inhibitory Concentration, the lowest concentration that inhibits visible growth. It is different from the concentration that kills all microbes.

Facts worth memorising in Pharmaceutical Microbiology

  • Fleming discovered penicillin from Penicillium, Jenner developed the smallpox vaccine and Sabin developed the oral polio vaccine.
  • Vibrio cholerae causes cholera, Salmonella typhi causes typhoid and Plasmodium causes malaria.
  • Bacteria divide by binary fission, and a plasmid is an extrachromosomal circular DNA.
  • A virus has either DNA or RNA, never both.
  • The BCG vaccine protects against tuberculosis, and HIV infection leads to AIDS.
  • Agar is the usual solidifying agent in culture media, and nutrient agar is a general-purpose medium.

How to use this quiz for revision

Make three small lists: scientist and discovery, organism and disease, and sterilisation and indicator. Test yourself by covering one side of each list. Then attempt this pharmaceutical microbiology MCQ quiz again. You will notice that your mistakes now come from one list only, and that is the one to revise next.

Frequently asked questions about pharmaceutical microbiology MCQ

Why is microbiology important for pharmacists?

Pharmacists need it for sterile products, antibiotics, vaccines and quality control of medicines.

Which topics are most important?

Sterilisation, staining, culture media, common pathogens, antibiotics and vaccines.

Does the quiz include sterilisation questions?

Yes. It includes autoclave and dry heat indicators, phenol coefficient and culture media.

Keep practising

If this pharmaceutical microbiology MCQ set felt comfortable, move on to the next subject. These pages will help:

For official syllabus details and regulations, refer to World Health Organization and CDC.

Written by the Noteskarts pharmacy education team. This quiz is for exam practice and learning only; always check your latest syllabus and standard textbooks for final answers.

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