Instrumental Methods of Analysis PDF Notes | B.Pharma 7 sem PDF Notes Noteskarts
Syllabus
Unit: 1
- UV Visible spectroscopy: Electronic transitions, chromophores, auxochromes, spectral shifts,
solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube,
Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
Applications- Spectrophotometric titrations, Single component and multi component analysis. - Fluorimetry: Theory, Concepts of singlet, doublet and triplet electronic states, internal and
external conversions, factors affecting fluorescence, quenching, instrumentation and
applications.
Unit: 2
- IR spectroscopy: Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations.
- Instrumentation- Sources of radiation, wavelength selectors, detectors – Golay cell,
Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications. - Flame Photometry- Principle, interferences, instrumentation and applications.
Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications. - Nephelo-turbidimetry- Principle, instrumentation and applications.
Unit: 3
Introduction to chromatography:
- Adsorption and partition column chromatography- Methodology, advantages, disadvantages and applications.
- Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.
- Paper chromatography- Introduction, methodology, development techniques, advantages, disadvantages and applications.
- Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications.
Unit: 4
- Gas chromatography – Introduction, theory, instrumentation, derivatization, temperature
programming, advantages, disadvantages and applications. - High performance liquid chromatography (HPLC)- Introduction, theory, instrumentation,
advantages and applications.
Unit: 5
- Ion exchange chromatography- Introduction, classification, ion exchange resins, properties,
mechanism of ion exchange process, factors affecting ion exchange, methodology and
applications. - Gel chromatography- Introduction, theory, instrumentation and applications.
- Affinity chromatography- Introduction, theory, instrumentation and applications.